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GFP expression by retinal cells during the initial culture period. The percentage of cells exhibiting GFP fluorescence increased markedly over the initial 36 days in culture. GFP-positive cells were quite rare in primary retinal isolates from fetal GFP-transgenic pigs, and remained below 10% of the total population until culture day 14. Subsequently, the percentage of GFP-positive cells increased rapidly, reaching 50% on day 22 and 80% on day 36, the latest time point examined.
