Table 2.
Transplantation of GFP-Transgenic CNS Progenitor Cells to the Retina of 4-Month-Old Allorecipients
| Pig no. | Survival time | Retinal pretreatment | Surviving GFP cells | Localization of GFP cells |
|---|---|---|---|---|
| A. | ||||
| 170 | 9 days | Laser | +++ | SR, V |
| 171 | 9 days | Laser + pressure | +++ | SR, V |
| 172 | 9 days | Pressure | +++ | SR, R, V |
| 168 | 3 weeks | Laser + pressure | +++ | SR, R, V |
| 173 | 3 weeks | Laser | ++ | SR |
| 174 | 3 weeks | Pressure | + | SR, V |
| B. | ||||
| 132 | 2 weeks | Laser | +++ | SR, Ro, R, V |
| 133 | 2 weeks | Scraped | ++ | C, SR, Ro |
| 134 | 2 weeks | Laser | +++ | SR |
| 175 | 3 weeks | Laser + pressure | +++ | SR, R, V |
| 128 | 5 weeks | Laser | +++ | SR, R |
| 129 | 5 weeks | Scraped | +++ | SR, R |
| 135 | 5 weeks | Scraped | +++ | SR, V |
| 176 | 5 weeks | Laser | +++ | SR, R |
| 177 | 5 weeks | Laser | ++ | SR |
| 178 | 5 weeks | Laser | +++ | SR, R |
| 130 | 10 weeks | Laser | + | SR |
| 131 | 10 weeks | Scraped | +++ | SR, R |
| 167 | 10 weeks | Laser | + | R |
| 169 | 10 weeks | Laser | +++ | SR, R |
| 179 | 10 weeks | Laser | +++ | SR |
Donor cells were derived from either (A) forebrain or (B) neural retina of fetal GFP-transgenic pigs.
Cell survival is estimated compared to number of injected cells as follows: +++ = is consistent with an order of magnitude decrement in cell number, ++ = an additional order of magnitude decrement, and += yet another order of magnitude decrement.
Abbreviations used: C = choroid, SR = subretinal, Ro = rosettes present, R = retina, V = vitreous.