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. Author manuscript; available in PMC: 2010 Nov 9.
Published in final edited form as: Prostate. 2009 Jul 1;69(10):1128–1141. doi: 10.1002/pros.20962

Fig. 1.

Fig. 1

Construction and characterization of PSMA-retargeted measles virus. A: Schematic representation of the parental MV-GFP and PSMA fully retargeted measles viral genomes. The 481Y → A and 533R → Amutations in Hablate CD46/SLAM interaction. The anti-PSMA (or CD38) single-chain antibody (scFv) is inserted at the COOH-terminal of mutated H followed by a six-histidine peptide (H6). B: Immunoblotting of MV-GFP, MVG-αPSMA and MVG-αCD38 virions using anti-H and anti-N antibodies. 5 ×104 TCID50 of each virus was loaded. The chimeric H glycoproteins of MVG-αPSMA and MVG-αCD38 (lanes 2 and 3) have higher molecular weights compared to that of MV-GFP (lane 1). C: One-step growth kinetics of MV-GFP, MVG-αPSMA and MVG-αCD38 on Vero-αHis cells.