Figure 3.

Evaluation of assay performance at different stringencies using hsPRS-v1 and hsRRS-v1. (a) The tradeoff between true and false positive rate at different stringencies of the tool-kit assays. For different applications, different thresholds may be used. (b) Y2H assay performance in different scoring protocols (one vs. two reporters) and different bait and prey expression levels (high vs. low copy plasmid, y-axis). 3-AT (3-amino-1,2,4-triazole) is a competitive inhibitor of the HIS3 gene product and was included to reduce background in one set of experiments.