Figure 9. Long-term protection generated by the F3sap vaccine.

Balb/c mice were vaccinated with NH36sap, F1sap, F2sap or F3sap at the indicated time intervals, through the sc route, followed by the intravenous challenge with L. chagasi amastigotes 28 days after the last immunization. Bars represent the mean ± SD of the individual parasite load in liver measured by LDU (one experiment, n = 3–4 mice). *p<0.05 significant differences from the saline controls and ○ from the F2sap vaccine.