Table 1. NH36 antibody epitope mapping.
Fragment | position | Sequence (aa) | antibody binding inhibition (%) |
F1 | 40–57 | N Q T L E K V T R N A R L V A D V A G | 0.00 |
F1–F2 | 94–108 | P E F K T K L D G R H A V Q L | 2.15 |
F2 | 114–126 | M S H E P K T I T L V P T | 0.46 |
F3 | 202–219 | A V Q K R V K E V G T K P A A F M L | 31.40 |
F3 | 228–239 | V Y E K E R N T Y A T V | 18.82 |
F3 | 278–291 | F R Y P R P K H C H T Q V A | 20.89 |
Percent of the antibody binding inhibition from sera of Leishmune vaccinated dogs by each of the predicted synthetic peptides in a competitive ELISA assay.