Table 2. Results of reference-guided assembly.
| N2473 | N2474 | N2173 | N2175 | |
| 5 infected, M | 5 infected, F | 1 of 5 infected, F | ||
| rRNA depletion | − | + | − | − |
| number reads mapped | 243 | 419 | 227 | 46 |
| number bases in assembly | 10,412 | 10,106 | 9,699 | 6,282 |
| % DENV-1 genome covered | 97.0% | 94.1% | 90.3% | 58.5% |
| number large contigs | 7 | 3 | 7 | 3 |
| total contigs | 7 | 5 | 8 | 15 |
| N50 contig size (nt) | 1,635 | 3,197 | 2,050 | 758 |
| largest contig size (nt) | 2,272 | 5,590 | 2,366 | 1,149 |
| average depth of coverage | 4.5 | 8.3 | 4.9 | 1 |
Each of the 5 datasets were assembled with GS Reference Mapper software using the DENV-1 genome as a reference. Statistics obtained for each of the 4 datasets containing DENV-1 specific reads are shown here. The N2187 control noninfected mosquito dataset was also mapped to the DENV-1 genome, but resulted in no assembled reads.