Table 3.
Oligonucleotide primers used in the construction of MATE1 reporter plasmids or AP-2rep plasmid and the electrophoretic mobility shift analysis
| Primers for MATE1 promoter sequencing | |
| Sense | 5’-GGG AGC ATG TTG CTC TAT CC-3’ |
| Antisense | 5’-AGG AGC TTC CAT GTG ACT CG-3’ |
| Primers for MATE1 promoter cloning | |
| First PCR (−543 to +15) | |
| Sense | 5’-AGT ATG CAA CAC CCT AAC CAG CA-3’ |
| Antisense | 5’-CCT CAG GAG CTT CCA TGT GAC T-3’ |
| Second PCR (−234 to −12) | |
| Sense (NheI site)1 | 5’-CTA GCT AGC GGT GCA GAG AGA GGT GCA A-3’ |
| Antisense (HindIII site)1 | 5’-CCC AAG CTT GCT GCG GCC GGG TAG-3’ |
| Primers for AP-2rep cloning | |
| Sense (NheI site)1 | 5’-GCT AGC ATG AAT ATC CAT ATG AAG AGA-3’ |
| Antisense (KpnI site)1 | 5’-GGT ACC GCT GGA CAG GTA GCA TTC CTC AC-3’ |
| Primers for EMSA | |
| Reference (g.−66T)2 | 5’-TGC GCG GTA CTC ACT GCC GGC C-3’ |
| Variant (g.−66C)2 | 5’-TGC GCG GTA CCC ACT GCC GGC C-3’ |
| Consensus AP-13 | 5’-AAG CCT GTG ACT CAG GAC CTG-3’ |
| Consensus AP-2rep4 | 5’-CTG GGG CAG TGG GAC TGG CA-3’ |