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Oxygen transfer rates of E. coli SG13009 pKK233-2 expressing wild type BFD in modified PanG mineral medium. Modified 250 mL shake flasks; filling volume: 10 mL; shaking frequency: 320 rpm; shaking diameter: 50 mm; temperature: 37°C; all OTRs are shown from 6 h to enlarge the relevant part of the graphs. (A) Different initial glycerol concentration (10 mg/L thiamine). (B) Different initial thiamine concentration (10 g/L glycerol).
