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ASMase is involved in α-TEA-induced accumulation of DR5, Fas, FADD and ceramide in cell surface membranes. Cellular localization of DR5, Fas and FADD, as well as ceramide were detected by immunofluorescent staining of paraformaldehyde fixed cells with primary antibodies to DR5, Fas, FADD, and ceramide followed by fluorescent labeled secondary antibody. All data are representative of three independent experiments.
