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. 2010 Nov 9;5(11):e15398. doi: 10.1371/journal.pone.0015398

Figure 5. C. jejuni 11168H infected-DC supernatants promote expansion of Th-17/Th-1 immunity.

Figure 5

PBMCs were enriched for CD4+CD45RO+ memory T cells and stimulated with anti-CD3, anti-CD28 coated beads for 5 days in the presence of supernatants taken from DCs cultured with medium only or with C. jejuni 11168H WT strain. (a) A representative flow cytometric plot of cultured T cells, stimulated with PMA and ionomycin for 3 hours in the presence of Brefeldin A and stained for intracellular IL-17A and IFNγ is shown. (left panel; T cells cultured in uninfected DC supernatants, right panel; T cells cultured in C. jejuni-infected DC supernatants). (b) Number of IL-17+IFNγ+ (left), IL-17+IFNγ− (middle) and IFNγ+IL-17− (right) cells as a percentage of CD4+ T cells, as in (a), n = 7. (c) T-cell derived cytokine [IFN-γ (n = 4; left), IL-17 (n = 4; middle) and IL-22 (n = 6; right),] protein quantified 5 days post-stimulation.