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. Author manuscript; available in PMC: 2011 Dec 10.
Published in final edited form as: Exp Cell Res. 2010 Jun 27;316(20):3406–3416. doi: 10.1016/j.yexcr.2010.06.011

Figure 4. N-WASP overexpression can rescue morphology defects of WASP deficiency.

Figure 4

Cells treated with a non-targeting shRNA (control), vector transfected shWASP cells (vector), or N-WASP overexpressing clones from shWASP (F6, E7, D3) were plated on coverslips. (A) Representative images show control (top), vector (middle) and N-WASP overexpressing clone E7 (bottom) stained for F-actin (red) and vinculin (green). Scale bar represents 10µm. Insets show a 2µm by 2µm square from the original image, highlighting the vinculin encircled cores of F-actin that distinguish podosomes in both control and N-WASP F6, but not present in vector expressing shWASP cells. (B) The fraction of cells that displayed migratory morphology was determined (n=6). (C) Elongation index was measured using ImageJ (n=5). At least 60 cells per experiment were analyzed for each determination. * p<0.05