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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Biomaterials. 2010 Oct 12;31(36):9422–9430. doi: 10.1016/j.biomaterials.2010.08.064

Fig. 2.

Fig. 2

Mineralizing MC3T3-E1 osteoblast cultures were incubated with VTK and pVTK at the indicated concentrations for 12 days followed by mineral quantification by (A) calcium content determination expressed as a percentage of untreated control cultures, and (B) von Kossa (silver nitrate) staining for mineral. Data are presented as means ± S.D. *p < 0.05; ***p < 0.001 from Student's t-test for statistical differences between the two peptides at a given dose. (C) Cell proliferation in osteoblast cultures treated with, or without, 200 μM pVTK and VTK as measured by MTT assay. a denotes statistical significance between Control and VTK at the given time point. b denotes statistical significance between Control and pVTK at the given time point.