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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Eur J Immunol. 2010 Sep 24;40(11):3226–3234. doi: 10.1002/eji.201040349

Figure 2. Positive selection is normal in KSR1−/− HY female mice.

Figure 2

Single cell suspensions of (A, B) thymocytes or (C) splenocytes from HY female wild-type or KSR1−/− mice were counted and stained with antibodies to CD4, CD8, CD3 and HY TCR. Cells were collected on a BD FACSCalibur and analyzed using Flowjo software. Representative FACS plots are shown either ungated (left) or gated on HY TCR+ cells (right) in (A). Cell numbers are representative of 4 mice per group and are shown as mean ± SD (B, C). Differences between groups are not significant (P > 0.05; unpaired Student’s t-test).