Figure 5. Negative selection in KSR1−/− AND mice is normal.

Thymi from AND TCR wild-type or KSR1−/− mice on an (A) H-2^k or (B) H-2^bxk haplotype were counted and made into single-cell suspension and stained with antibodies to CD4, CD8, CD3 and Vα11. Data were collected using a BD FACSCalibur and analyzed using Flowjo software. Representative FACS plots are shown either ungated (left) or gated on the AND TCR⁺ cells (right) in (A). Cell numbers shown are for analyses on the H-2^k haplotype and are representative of 4 mice per group and are shown as mean ± SD (C). Differences between groups are not significant (P > 0.05; unpaired Student’s t-test).