Table 2.
Listing of New EEHV DNA POL PCR Primers Used
| Description | Number | Orient | Sequence (5'–3') | Pairing and Product Size (bp) | |||||
|---|---|---|---|---|---|---|---|---|---|
| Round | 1A | 2A | 2B | 3A | |||||
| Specific EEHV3/4: | |||||||||
| 1. EB4P | A1 | 6719 | R | -CGTTGAAGGTGTCGCAGAT - | 390 | 250 | 130 | ||
| 2. EB4P | B1 | 7400 | L | -CAGCATCATCCAGGCCTACAAC - | 390 | ||||
| 3. EB4P | B2 | 6720 | L | -ATCCTGGCGCAGCTGCTGAC - | 250 | ||||
| 4. EB4P | B3 | 6721 | L | -CTCACCTGCAACGCCGTCTA - | 130 | ||||
| PAN EEHV and Specific EEHV6: | |||||||||
| 5. PAN-EBP | A1 | 6710 | R | -ACAAACACGCTGTCRGTRTCYCCRTA- | 500 | 250 | 230 | ||
| 6. PAN-EBP | B1 | 6711 | L | -GTATTTGATTTYGCNAGYYTGTAYCC- | 500 | 470 | |||
| 7. PAN-EBP | B2 | 6712 | L | -TGYAAYGCCGTNTAYGGATTYACCGG- | 250 | 220 | |||
| 8. EB6P | A2 | 7584 | R | - CATCGATTTTGAACTTCTCATGGTC- | 470 | 230 | 220 | ||
| Specific EEHV5: | |||||||||
| 9. EB5P | A1 | 7483 | R | -CTACATCTATACAGAACTTTCC- | 500 | 230 | |||
| 10. EB5P | B1 | 7484 | L | -GTACCTTAGTTACGGACGAGAC- | 500 | 480 | 210 | ||
| 11. EB5P | A2 | 7424 | R | -CGACAGCACACTCCTTTAACAC- | 480 | 210 | |||
| 12. EB5P | B2 | 7485 | L | -CGCTGTATATGGATTTACCGG- | 230 | ||||
| PAN EGHV and Specific EGHV2: | |||||||||
| 13. PANEGP | A1 | 6784 | R | -GTGGKGACTTTGCYAGCCTSTACCC- | 330 | ||||
| 14. PANEGP | B1 | 6788 | L | -ACMCCNGTRAATCCRTASAC- | 330 | 310 | |||
| 15. PANEGP | A2 | 6785 | R | -CCCMAGYATTATWCAGGCMCA- | 310 | 310 | |||
| 16. EG2/3P | A3 | 6786 | R | -CCCCAGCATTATACAGGCACA- | 300 | ||||
| 17. EG2P | B3 | 6787 | L | -ACCCCTGTAAATCCATACACTG- | 300 | 310 | |||
| PAN EGHV and Specific EGHV3: | |||||||||
| 18. PANEGP | A1 | 6784 | R | -GTGGKGACTTTGCYAGCCTSTACCC- | 550 | 330 | |||
| 19. PANEGP | B4 | 7489 | L | -GTCRGTGTCYCCGTAGAYNAC- | 550 | ||||
| 20. PANEGP | A2 | 6785 | R | -CCCMAGYATTATWCAGGCMCA- | 310 | ||||
| 21. EG3P | B5 | 7486 | L | -CTCCCGTAAATCCATACACAGC- | 330 | 310 | |||