Table 1.
RSC remodeling rate constants
Rate Constants (min−1) | 1 mM ATP | 20 μM ATP | ||
---|---|---|---|---|
k1 | k2 | k1 | k2 | |
Repositioning Alone | ≥ 10 | ≥ 10 | ||
Dimer Transfer | 8.2 ± 0.14 | 0.027 ± 0.00057 | 1.9 ± 0.098 | 0.41 ± 0.028 |
Repositioning Under Dimer Transfer Reaction Conditions | ≥ 10 | ≥ 9 | ||
Octamer Transfer | 3.7 ± 1.4 | 0.033 ± 0.0084 | 0.66 ± 0.062 | 0.082 ± 0.015 |
Repositioning Under Octamer Transfer Reaction Conditions | ≥ 10 | 0.80 ± 0.017 | ≥ 9 | 0.83 ± 0.043 |
Each value is the average of three independent experiments ± standard error of the mean. k1 and k2 are the rate constants of the first and second phase, respectively.