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. Author manuscript; available in PMC: 2011 Nov 16.
Published in final edited form as: Biochemistry. 2010 Oct 21;49(45):9706–9714. doi: 10.1021/bi100509s

Fig. 5.

Fig. 5

Comparison of the binding affinity of Nuc-1,2,3,4-RGG, Nuc-3,4-RGG, and Nuc-RGG for the c-MYC G-quadruplex. 10,000 cpm of radiolabeled oligomer was assembled into a G-quadruplex structure and incubated with Nuc-1,2,3,4-RGG (A), Nuc-3,4-RGG (B), or Nuc-1,2,3,4-RGG (C) at the indicated concentrations in a 20-μL reaction containing 10 mM Tris-HCl, pH 7.4, 1 mM EDTA, 1 mM DTT, 50 ng/μL poly(dI-dC), 4 μg/mL BSA, and 25 mM KCl for 1 h at room temperature.