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. Author manuscript; available in PMC: 2010 Nov 10.
Published in final edited form as: Front Biosci. 2008 May 1;13:6237–6254. doi: 10.2741/3151

Figure 7.

Figure 7

Tetramerization of Stat5 protein variants. Tetramer formation of the indicated Stat5a variants (61) was analyzed using 293T cell extracts stimulated with Epo (50 U/ml) using the IL-2R-alpha chain tetramer element (upper part). Four different tetramer DNA elements (CIS-AB, 2xRE; CIS-CD, 2xRE; IL-2R-alpha, 2xRE beta-casein, 2xRE) were used for incubation with a Stat5a extract from Epo-activated cells to demonstrate dimer and/or tetramer formation (lower part). “#” indicates formation of both dimers and tetramers.