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. Author manuscript; available in PMC: 2010 Nov 10.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Sep 13;376(3):472–477. doi: 10.1016/j.bbrc.2008.09.009

Figure 3. ABC transporter antibody treatment of spermatozoa affects cholesterol efflux and inhibits in vitro fertilization.

Figure 3

Figure 3

A, levels of spermatozoa cholesterol efflux to apoA-I in the absence or presence of antibodies to ABCA1, ABCA7 or ABCG1. B, levels of spermatozoa cholesterol efflux to BSA in the absence or presence of antibodies to ABCA1, ABCA7 or ABCG1. As controls, spermatozoa were incubated with IgG without apoA-I (Control) or with apoA-I or with BSA alone. * indicates that when compared to both controls the difference is significant (p<0.05). ** indicates that when compared to the IgG control without apoA-I or BSA the difference is significant (p<0.05). C, epididymides spermatozoa were treated with control antibody (IMA32) or antibodies to ABCA1, ABCA7 and ABCG1 (0.1µg IgG/ml), either alone or in combination and incubated with groups of 10 oocytes. Fertilization was assessed 18h later. The plotted values are means from assessments of the indicated number of oocytes for each treatment. The difference between a and b or b and c are significant at the p<0.05 level.