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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Biochem Pharmacol. 2010 Jun 23;80(12):2050–2056. doi: 10.1016/j.bcp.2010.06.019

Figure 5.

Figure 5

(A) Dose dependent inhibition of [3H]-LOS transfer to His tagged tCD14. This capture assay shows the dependency between molecule 8 concentration and the inhibition of [3H]-LOS-sCD14 complex formation. (B) Molecule 8 does not inhibit [3H]-LOS transfer from CD14 to MD2. [3H]-LOS–MD-2 complex formation is not inhibited if MD-2 is pre-incubated with molecule 8 suggesting that the compound could not compete with LOS for MD-2 binding. Data shown are representative of 3 experiments, each in duplicate, and are expressed as mean of percent of radioactivity captured in comparison with total radioactivity measured ± SEM. The asterisks indicate a significant statistical difference (P<0.01; ANOVA, Dunnett’s test) between data in the analyzed group and reference data (sample without molecule 8).