Table IV.
Ex Vivo Tissue Diffusion
| Parameters to standardize | • Animal species and age |
| • Fed/fast status of animal | |
| • Anesthesia regimen | |
| • Stripped or unstripped tissue | |
| • Intestinal region | |
| • Time to equilibrium | |
| • Diffusion buffer composition, osmolarity, and pH | |
| • Monitoring of viability and integrity | |
| • Oxygenation of buffer and mixing process | |
| • Sink conditions and sampling method | |
| • Drug analysis and P app calculation | |
| Acceptance criteria | • Measure of tissue viability/integrity |
| • Potential difference, tissue resistance, and/or short circuit current | |
| • P app of non-absorbable marker | |
| • P app of HP and LP markers | |
| • P app of active transport marker |