Fig. 3.

Influence of culture medium on TEER. a TEER values across endothelial cells after 6 days in co-culture. Data are presented as mean values from 6 different co-culture setups (N = 6) each containing 3–6 filter inserts with the different media (n = 3–6). Error bars represent standard deviations of the averages from the different cultivations. DM Differentiation medium. b Apparent mannitol permeability across the endothelial cells after 6 days in co-culture. Data are single determinations from 6 filter inserts cultured in differentiation medium (filled square) and 6 filter inserts cultured in differentiation medium + HEPES (filled triangle)