Table III.
Overview of Isolation Outcomes of the Different Batches. Impurities were Defined as any Cell Growth that did not Display Endothelial Cell Morphology. Transendothelial Electrical Resistance (TEER) Values are Average Values of 6–12 Filter Inserts (n = 6–12) from 1 to 5 Different Experiments (N = 1–5)
| Isolations of bovine brain capillary endothelial cells. | ||||
|---|---|---|---|---|
| Batch | Age of calf (months) | Impurities (%) | Yield upon passage (million cells) | TEER day 6 in DM (Mean ± SD, Ω ⋅ cm2) |
| A | Unknown | 0–2 | 2 | 261 ± 26 |
| B | 15 | 1–3 | 1.8 | 337 ± 62 |
| C | 12 | 0–2 | 2 | 505 ± 110 |
| D | 32 | 10 | 0.45 | – |
| E | 14 | 0–2 | 1.1 | 760 ± 46 |
| F | 30 | 1–5 | 0.65 | – |
| G | 30 | 5–10 | 0.2–0.3 | – |
DM differentiation medium