Table I.

Findings of Ten Cytokines in Ten Individuals 24 h after Insertion of a Cutaneous Microdialysis Catheter Membrane

Subjects	IL1b		IL6		IL8		GM-CSF		TNFa		IL2		IL4		IL5		IL10		INFg
	IF	M	IF	M	IF	M	IF	M	IF	M	IF	M	IF	M	IF	M	IF	M	IF	M
1	0	17.7	++	141.3	++	134	0	14.5	+++	8.2	+	7.9	0	10.6	ND	10.5	0	0	0	0
2	0	0	0	40.9	++	23.3	0	0	+	0	0	0	0	0	ND	0	0	0	0	0
3	+++	22.8	++	820.7	++	504.2	+	20.9	0	8.2	+	0	0	0	ND	8	0	6	0	0
4	+++	0	+++	24.7	++	252.0	+++	7.7	++	0	+++	12.6	++	0	ND	0	0	0	0	0
5	++	12.2	++	116.8	++	154.9	ND	7.7	0	8.2	ND	0	ND	0	ND	0	ND	0	+	0
6	0	0	+	436.8	++	778.6	+	0	0	0	++	14.7	0	0	0	0	+++	0	+	0
7	0	27.4	+	702.6	++	296.8	0	0	+	0	0	5.5	0	0	+	0	0	0	0	0
8	0	19.7	0	699.5	++	982.1	0	0	0	0	0	5.5	0	0	0	0	0	0	++	0
9	0	0	0	25.2	++	1040.3	0	0	0	0	0	5.5	0	0	0	0	+	0	0	0
10	0	6.2	+++	225.8	++	220.2	0	0	++	0	0	0	0	0	0	0	0	0	+	0
Analysable IF and Ma	10	10	10	10	10	10	9	10	10	10	9	10	9	10	5	10	9	10	10	10
Total positive IF and Mb	3	6	7	10	10	10	3	4	6	3	4	6	1	1	1	2	2	1	4	0
Analysable pair comparisonsc	10		10		10		9		10		9		9		5		9		10
Total individual concordance (%)d	50		70		100		77		50		55		77		80		66		60

IF staining was performed on skin biopsies taken from the site of the catheter membrane. A semiquantitative scale was chosen: 0 = no cellular staining, + = 5 cells or less stained, ++ = between 5 and 20 cells, +++ = more than 20 cells. Three to four viewfields 37 × 104 μm² were evaluated at ×200 magnification. ND = technical obstructions. The cytokine concentrations in the microdialysis (M) samples (in pg/ml) are from samples collected the hour before biopsy. Concentrations that did not exceed the lowest standard concentration are noted as 0

^aNumber of evaluated subjects

^bIF = Number of positive immunofluorescence findings. M = Number of subjects who had cytokine levels above the lowest standard concentration in the last microdialysis sample

^cNumber of analysable observations of both immunofluorescence and cytokine concentrations in the same individual

^dPer cent concordance of the methods; number of evaluated positive or negative findings in agreement between IF + M divided by the number of paired comparisions × 100