Table I.

Identification of In Vivo Proteolytic Points of AMG531 by nanoLC-MS in Rat Samples at 24 h

Peaka	Average mass	∆m meas b	Proteolysis assignment	∆m theo b	% Errorc
1	26,975	2,718	Q241/W242	2,689	1.1
2	28,522	1,171	P259/T260	1,167	<1
3	28,610	1,080	T260/L261	1,066	1.3
4	29,006	687	Q263/W264	669	2.7
5	29,192	501	W264/L265	482	3.9
6	29,693	0	Parent	0	N/A

N/A not applicable

^aPeak numbers based upon annotation in Fig. 3

^b∆m _meas and ∆m _theo are the measured and theoretical mass differences between metabolite and parent

^c