Table IV.

Freeze-drying Recipe Used for 100 mg/mL Trehalose Solution

	1	2	3
Freezing step
Shelf setpoint [°C]	+5	−5	−40
Ramp rate [°C/min]	1	1	1
Time [min]	30	30	90
Primary/Secondary drying
Shelf setpoint [°C]	0	+40
Ramp rate [°C/min]	1	0.1
Time [min]	4000	240
Vacuum setpoint [mTorr]	100	100