Table V.

Freeze-drying Recipe Used for the 50 mg/mL Mannitol/Sucrose Mixture

	1	2	3	4	5
Freezing step
Shelf setpoint [°C]	+5	−5	−40	−15	−40
Ramp rate [°C/min]	1	1	1	1	1
Time [min]	30	30	0	180	120
Primary/Secondary drying
Shelf setpoint [°C]	−28	−3	+1	−5	+40
Ramp rate [°C/min]	0.5	0.5	0.5	0.5	0.3
Time [min]	57	57	234	354	240
Vacuum setpoint [mTorr]	85	85	85	85	85

Note that the primary drying phase of the recipe was automatically generated by the SMART Freeze Dryer software (16).