Figure 4.
ILK Regulates Organization and Stability of the MT Network
(A) Control and ILK-K5 keratinocytes stained for tubulin and F-actin. Note disorganized MT network and lack of MT tip association with cortical actin (arrow) in ILK-K5 cells. Scale bars, 10 μm.
(B) Control and ILK-K5 keratinocytes stained for acetylated tubulin and α tubulin. Scale bars, 50 μm.
(C) Reduced tubulin acetylation confirmed by western blotting.
(D) Quantification of acetylated tubulin levels (average ± SD; n = 3).
(E) Control and ILK-K5 keratinocytes treated with taxol and analyzed for acetylated tubulin.
(F) Control and ILK-K5 were treated with 1 μM nocodazole for time points indicated, after which cells containing more than 10 MTs were quantified (mean ± SEM; n = 3).
(G) Dynamics of MTs analyzed by spinning-disc microscopy in control and ILK-K5 keratinocytes expressing EGFP-tubulin. Images are snapshots from Movie S4 acquired at 2 s/frame. Right panels show an enlargement of the area indicated by the white rectangle. Arrowheads indicate stable MTs. Scale bars, 10 μm.