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. Author manuscript; available in PMC: 2010 Nov 10.
Published in final edited form as: J Surg Res. 1990 Jul;49(1):18–22. doi: 10.1016/0022-4804(90)90104-a

FIG. 1.

FIG. 1

Tracing from Hitachi spectrophotometer. The bottom line is the standard print out of the absorbance for 220–300 nM. The top line is the second derivative of the absorbance at each wavelength and shows how a “shoulder” in the standard printout is transformed into a distinct peak at 242 nM (cis, trans diene).