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The H7N7 NL/03 ca virus without the multibasic amino acid motif in the HA cleavage site requires trypsin for plaque formation in CEFs
| Virus | Amino acid sequence near the HA cleavage siteb | Titer in CEF cells (log10 PFU/ml) |
|
|---|---|---|---|
| With trypsin | Without trypsin | ||
| H7N7 NL/03 wt | PEIPKRRRR↓GLF | 8.6 | 8.6 |
| H7N7 NL/03 ca | PEIPK--GR↓GLF | 7.4 | <1.0a |
Lower limit of detection.
↓ indicates the site of cleavage of the HA into the HA1 and HA2 domains.
