TABLE 1.
Concentration resulting in 50% inhibition of HCVcc entry
| Virus | 50% neutralization concna (μg/ml) of: |
||||||
|---|---|---|---|---|---|---|---|
| Antibodies from sera positive for indicated HCV genotype |
MAb 3/11 | Lectin CV-Nb | CD81-LEL | ||||
| 1a | 1b | 2 | 4 | ||||
| Wild type | 3.9 (1.0) | >10 (1.0) | 10 (1.0) | >10 (1.0) | >10 (1.0) | 0.3 (1.0) | 4.3 (1.0) |
| HCVcc mutant | |||||||
| E2N1 | 1.4 (2.8) | 1.3 (>7.7) | 1.3 (7.7) | 0.7 (>14.3) | 0.4 (>25.0) | 0.2 (1.5) | 0.6 (7.2) |
| E2N2 | 0.6 (6.5) | 0.7 (>14.3) | 0.7 (14.3) | 0.5 (>20.0) | 0.4 (>25.0) | 0.4 (0.8) | 0.5 (8.6) |
| E2N4 | 0.7 (5.6) | 0.7 (>14.3) | 0.7 (14.3) | 0.6 (>16.7) | 0.6 (>16.7) | 0.3 (1.0) | 0.6 (7.2) |
| E2N5 | >10 (<0.4) | >10 (ND) | >10 (<1.0) | >10 (ND) | >10 (ND) | 0.5 (0.6) | >10 (<0.4) |
| E2N6 | 0.6 (6.5) | 0.6 (>16.7) | 0.7 (14.3) | 0.5 (>20.0) | 2.8 (>3.6) | 0.4 (0.8) | 0.5 (8.6) |
| E2N9 | 4.3 (0.9) | 2.9 (>3.4) | 8.1 (1.2) | 2.2 (>4.5) | >10 (ND) | 0.5 (0.6) | >10 (<0.4) |
| E2N11 | 1.5 (2.6) | 1.0 (>10.0) | 1.6 (6.3) | 0.8 (>12.5) | 2.4 (>4.2) | 0.5 (0.6) | 2.5 (1.7) |
a
Values in parentheses are differences (n-fold) from the 50% neutralization concentration of the wild-type. ND, not determined.
b
CV-N, cyanovirin-N.