FIG. 3.
3Cpro expression induced PML cleavage and degradation. (A) CHO-PMLIII cells were infected with VV, VV-P1, VV-P2, or VV-P3 at an MOI of 1 for 8 h. (B) CHO-PMLIII cells were infected with VV, VV-3AB, VV-3C, and VV-3D at an MOI of 1 for 8 h. (C) CHO-PMLIII cells were infected with VV or VV-3C at an MOI of 1 for 12 h (lanes 2, 3, and 4) or 16 h (lanes 5 and 6); extracts from CHO cells transfected with pcDNA 3C vector were used as a control for 3Cpro expression (lane 1). Total cell extracts were analyzed by Western blotting using anti-PML, anti-actin, or anti-3C antibodies. The unmodified form of PML is indicated by an arrowhead, and the cleavage products are indicated by arrows.