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. 2010 Sep 8;84(22):11634–11645. doi: 10.1128/JVI.01321-10

FIG. 7.

FIG. 7.

(A) EMCV infection increased PMLIII and PMLIII SIM SUMOylation. U373MG cells stably expressing PMLIII, PMLIII SIM, or PMLIII 3KR were infected with EMCV at an MOI of 5 for 2 h. (B) EMCV-induced PML degradation necessitated its covalent but not its noncovalent binding to SUMO. U373MG cells stably expressing PMLIII, PMLIII SIM, or PMLIII 3KR were infected with EMCV at an MOI of 5 for 8 h. The different cell extracts were analyzed by Western blotting and revealed by rabbit anti-PML or anti-actin antibodies. The unmodified form of PML is indicated by an arrowhead.