Skip to main content
. 2010 Sep 8;84(22):11709–11717. doi: 10.1128/JVI.01429-10

FIG. 3.

FIG. 3.

Inhibitors of clathrin-mediated endocytosis block the entry of RRV into RFs. (A and B) RFs were infected with RRV-luciferase in the presence of different concentrations of chlorpromazine (A) or dextrose (B). Luciferase activities in the culture supernatants were monitored at the indicated time points and were compared with those of control cells infected with RRV-luciferase in parallel without inhibitor treatment. (C) Inhibitors of clathrin-mediated endocytosis blocked the entry of Alexa Fluor 488-transferrin, but not that of Alexa Fluor 488-CTB, into RFs. (D) Inhibitors of clathrin-mediated endocytosis had no effect on cell viability. RFs were treated with inhibitors of clathrin-mediated endocytosis under the conditions used for the virus infection assay and were evaluated for viability by staining with PI and DAPI.