TABLE 1.
Primers used to amplify the coding region of Chilean isolates ISAV752_09 and ISAV901_09
| Segment | Primer sequence (5′-3′)a |
|
|---|---|---|
| Forward | Reverse | |
| 1 | CACCATGGACTTTATATCAGAAAACAC | TTAAACACCATATTCATCCATCAGGT |
| 2 | CACCATGGAAACTCTAGTAGGTGGG | TCAAACATGTTTTTCTTCTTAATCA |
| 3 | CACCATGGCCGATAAAGGTATG | TCAAATGTCAGTGTCTTCCTCCT |
| 4 | CACCATGGATAACCTCCGTGAA | TTATTGGGTACTGACTGCAATTTTC |
| CATGCCATGGATAACCTCCGTGAATGCATAAACC* | CCGCTCGAGTCATTGGGTACTGACTGCAATTTTC* | |
| 5 | CACCATGGCTTTTCTAACAATTT | TTATCTTCTAATGCATCCCCACAG |
| 6 | TTAAGCAACAGACAGGCTCGATG | CACCATGGCACGATTCATAATTT |
| 7 | CACCATGGATTTCACCAAAGTGTA | TTAATTCTCATTACAAATGTATTTTTCAAC |
| 8 | CACCATGAACGAATCACAATGGA | TTATTGTACAGAGTCTTCCAATTGGTC |
| CATGCCATGGACGAATCACAATGGATAC* | CCGCTCGAGTTATTGTACAGAGTCTTCCAATTG* | |
a
*, Primers specific for ISAV901_09.