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. 2010 Sep 15;84(22):11790–11801. doi: 10.1128/JVI.00916-10

FIG. 1.

FIG. 1.

ANDV and SNV elicit minimal and/or delayed ISG56 and MxA gene transcription in infected A549 and Huh7-TLR3 cells. (A) Induction of ISGs in A549 cells infected with Sendai virus (SeV) 4 h postinfection and Huh7-TLR3 treated with poly(I:C) 4 h posttreatment. (B) A549 and Huh7-TLR3 cells were infected with ANDV or SNV (MOI = 0.03). Cellular RNA was collected at 1, 2, and 3 dpi and analyzed for ISG56 and MxA mRNA levels by qRT-PCR using a SYBR green-based 2-step RT-PCR on a Corbett Rotor-gene 6000 system (values are means ± standard errors of the means [SEM] for two experiments). (C) TaqMan detection of intracellular levels of ANDV or SNV S segment in A549 and Huh7-TLR3 cells using TaqMan probes on a Corbett Rotor-gene 6000 system. Total RNA was collected at 1, 2, and 3 dpi. Standards were based on serial dilutions of known ANDV or SNV NP plasmid copy numbers, and samples were standardized by normalizing total input RNA per sample to 200 ng.