FIG. 6.

Conformation stability assay of PrP^res from frontal cortices of vCJD and MM1 sCJD cases. Western blot analysis of PrP^res from vCJD and sCJD MM1 brain samples treated with increasing concentrations of GdnHCl (0 to 4 M, as indicated) and then subjected to proteinase K digestion at the concentration of 20 μg/ml for 1 h at 37°C (+) or allowed to remain undigested. After methanol precipitation of the protein, samples were analyzed by Western blotting using the anti-PrP MAb 3F4. Molecular mass markers (M) are shown, and they are labeled with their molecular mass in kDa. The result shown is representative of two independent experiments, with samples from two different cases of vCJD and sCJD (MM1).