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Western blot analysis using γH2AX and pChk2 antibodies in normal mucosa, adenoma, early carcinoma and advanced carcinoma. The lysates from HL60 cells treated with camptothecin were used as a positive control. γH2AX and pChk2 expression levels were lowest in normal mucosa and gradually increased with tumor progression. β-actin served as a control for the amount of protein loading. PC: positive control.
