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. Author manuscript; available in PMC: 2011 Dec 10.
Published in final edited form as: Exp Cell Res. 2010 Oct 13;316(20):3478–3488. doi: 10.1016/j.yexcr.2010.09.015

Fig 4. Ciglitazone does not reduce DHT- mediated increases in PSA protein or mRNA within C4-2 cells.

Fig 4

(A) Whole cell lysates were prepared from LNCaP and C4-2 cells treated with ethanol (DHT-, Cig-), DHT (1 nM), Ciglitazone (45 μM), or DHT + Ciglitazone. PSA protein levels in treated cells were then detected using Western blot analysis. The blots were stripped and reprobed for β-actin to confirm equal loading of the gels. (B) Total RNA extracted from LNCaP and C4-2 cells treated with ethanol (DHT-, Cig-), DHT (1 nM), Ciglitazone (45 μM), or DHT + Ciglitazone. PSA mRNA levels were detected using qRT- PCR. The amount of PSA gene product in each sample was calculated using the ΔΔCt method and normalized to the amount of 18s control. Each bar represents the mean of two independent readings. A representative experiment is shown.