In (A) C4-2 cells were transfected with the PSAE- luciferase reporter, the CMV β-galactosidase plasmid, and either a non- specific control or PPARγ SMARTpool siRNA. In (B) LNCaP cells were transfected with the PSAE- luciferase reporter, the CMV β-galactosidase plasmid, and either a pcDNA 3.1 empty vector or PPARγ cDNA expression vector. For both panels, following transfection, both C4-2 and LNCaP cells were treated for 24 hours with the indicated drugs. Luciferase activity in treated cells was measured and normalized to β-galactosidase activity. Each bar represents the mean ± SD of three wells. *, P< 0.05 compared to DHT alone. In parallel wells, PPARγ and actin protein levels were measured using Western blot analysis (insets). A representative experiment is shown.