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Diffusion constant of DHPE and PGPE in a DOPC/cholesterol bilayer as a function of cholesterol content, determined by single molecule tracking. DOPC bilayers containing up to 50% cholesterol were labeled with PGPE-Alexa647 (full circles) and DHPE-Bodipy (open circles). Experiments were performed at 37 °C (A) or 25 °C (B).
