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. Author manuscript; available in PMC: 2010 Nov 10.
Published in final edited form as: J Immunol. 2008 Sep 1;181(5):3609–3619. doi: 10.4049/jimmunol.181.5.3609

Table II.

αMβ2 clustering is required for submission of prosurvival signala

Inhibitors PMN Early/Late Apoptosis (% Annexin V+ cells)
No Ligand Fg Plg P2-C P1
No inhibitor 65.4 ± 15 22.4 ± 5 22.3 ± 6 20.1 ± 4 56.1 ± 13
MβCD 55.6 ± 17 59.3 ± 15 75.0 ± 13 75.6 ± 17 66.3 ± 18
MLCK inhibitor peptide 18 70.9 ± 14 84 ± 24 68.1 ± 16 82.0 ± 22 65.3 ± 17
H7 77.6 ± 18 62.3 ± 16 79.2 ± 18 78.6 ± 17 60.1 ± 14
BDM 98.9 ± 20 98.7 ± 24 99.2 ± 24 97.8 ± 22 85.4 ± 20
Cytochalasin D 58.9 ± 18 55.5 ± 18 66.6 ± 15 77.4 ± 16 66.07 ± 15
Calpain inhibitor III 97.3 ± 24 88.9 ± 23 97.1 ± 22 74.7 ± 19 94.5 ± 22
a

PMNs were incubated with inhibitors of integrin clustering pathways and various αMβ2 ligands (proteins at 1 μM, peptides at 100 μM) as indicated for 16 h at 37°C. The data represent means ± SEM (n = 5).