. Author manuscript; available in PMC: 2010 Nov 10.

Published in final edited form as: J Immunol. 2008 Sep 1;181(5):3609–3619. doi: 10.4049/jimmunol.181.5.3609

Table VI.

The role of the α_Mβ₂ cytoplasmic tails and integrin activation in K562 survival^a

α_Mβ₂ Variants	K562 Early/Late Apoptosis (% Annexin V⁺ Cells)
α_Mβ₂ Variants	No Ligand	Fg	Plg	P2-C	P1	P2-Cscr	Ang(1–4)
α_Mβ₂	72 ± 14	28 ± 5	29 ± 4	26 ± 4.5	82 ± 6	71 ± 17	71 ± 15
α_Mβ₂(728Δ)	76 ± 15	80 ± 10	79 ± 10	74 ± 10	76 ± 11	76 ± 15	74 ± 16
α_M(1119Δ)β₂	73 ± 11	80 ± 9	81 ± 8.5	80 ± 16	77 ± 9	78 ± 12	78 ± 14
α_M^{(Ile316→Gly)}β₂	77 ± 12	19 ± 2.5	16 ± 2.1	15 ± 3	79 ± 7	76 ± 13	80 ± 9
α_M^{(D294C/Q311C)}β₂	78 ± 10	20 ± 3	18 ± 3.2	17 ± 3.5	80 ± 8	79 ± 10	76 ± 10
α_M/α_Lβ₂	77 ± 12	80 ± 7.7	76 ± 13	81 ± 8	81 ± 6	80 ± 8	80 ± 5
Mock	81 ± 8	72 ± 13	78 ± 10	76 ± 12	70 ± 12	77 ± 6	78 ± 8

K562 cells 48 h after transfection with the indicated α_Mβ₂ constructs were incubated in the presence or absence of various α_Mβ₂ ligands (proteins at 1 μM, peptides at 100 μM) for 16 h at 37°C. The data represent means ± SEM (n = 5). Integrin expression levels were monitored by FACS and were similar.