Figure 2. Microtubule Targeting Drugs Inhibit Ligand-dependent AR Transcriptional Activity.
LNCaP cells were treated with DHT (1nM) in the presence or absence of Nocodazole (5ug/ml) or Paclitaxel (1μM). Panel A; PSA mRNA expression was determined by realtime PCR. Panel B; PSA protein levels were assessed by Western blotting and relative expression was quantitated (lower panel). Panel C; The AR transcriptional activity in response to microtubule-targeting drugs was determined using the ARE luciferase reporter vector in LNCaP cells.