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. Author manuscript; available in PMC: 2010 Nov 10.
Published in final edited form as: Dev Biol. 2004 Sep 15;273(2):257–275. doi: 10.1016/j.ydbio.2004.06.002

Fig. 7.

Fig. 7

The effects of Shh on prostatic Fgf10 expression in the developing prostate gland. (A) Whole-mount ISH for ptc transcript in the pnd 0 UGS-prostate complex following culture for 24 h in the absence (left) or presence (right) of anti-Shh antibodies. Ptc expression was lost following treatment with anti-Shh antibodies, indicating that they functionally blocked Shh action. (B) Whole-mount ISH for Fgf10 transcript in the pnd 0 UGS-prostate complex following culture for 24 h in the absence (left) or presence (right) of anti-Shh antibodies. Blockade of Shh action markedly increased Fgf10 expression in all prostate lobes. Identical results were obtained with three separate sets of tissues. (C) Fgf10 expression by wmISH in the pnd1 LP and VP 1 day after placement of BSA beads on the prostate surface. (D) Fgf10 expression was reduced in the LP and VP 1 day after placement of SHH beads along the prostate surface. The tissue in D was processed together with the tissue in C to allow comparison of stain intensity. Similar results were obtained from three separate tissue sets. (E) Fgf10 expression by wmISH in the VP 3 days after implantation of a BSA bead (top) or a SHH bead (bottom) in the distal mesenchyme. Fgf10 expression was reduced in the immediate vicinity of the SHH bead but was unaffected by the BSA bead. (F) The VP shown in E (bottom) before wmISH shows inhibition of ductal growth and branching in the immediate vicinity of the SHH bead. Bar in A–F = 200 µm.