Table 2.

Inhibition of NOS isozymes by synthetic compounds 8–34.

Compounds	Ki (μM)a			Selectivityb
	nNOS	eNOS	iNOS	n/e	n/i
L-NNAc	0.57	0.75	4.55	1.3	8
(±)-8	47.94	121.62	609.43	2.5	13
(±)-9	3.98	172.91	437.76	43	110
(±)-10	304.96	849.21	2,522.8	3	8
(±)-11	13.47	1,696	651.85	126	48
(±)-12	6.94	340.83	25.24	49	4
(±)-13	15.68	241.7	308.1	15	20
(±)-14	14.45	2,906	166.1	201	11.5
(±)-15	13.86	1,453	298.55	105	21.5
(±)-16	20.57	1,453	416.61	71	20
(±)-17	12.85	625.42	234.06	49	18
(±)-18	20.93	460.16	253.43	22	12
(±)-19	17.92	108.69	209.01	6	12
(±)-20	24.4	170.52	285.54	7	12
(±)-21	0.61	262.74	32.03	431	52.5
(±)-22	0.53	118.34	44.85	223	85
(±)-23	0.25	95.18	44.11	380	176
(±)-24	0.74	61.19	40.42	83	55
(±)-25	0.87	78.75	27.35	90.5	31
(±)-26	0.74	108.55	58.72	147	79
(±)-27	0.66	201.61	83.9	305.5	127
(±)-28	0.414	63.22	23.76	153	57
(±)-29	0.483	76.13	44.23	158	92
(±)-30	0.589	16.47	20.46	28	35
(±)-31	1.891	58.14	75.36	31	40
(±)-32	0.088	123.90	18.17	1408	206.5
(±)-33	0.119	44.09	22.59	370.5	190
(±)-34	0.048	49.07	26.59	1022	554

^aThe apparent K_i values are represented as the mean of two or more independent experiments preformed in duplicate with five or six data points each and correlation coefficients of 0.88–0.99. The experimental standard deviations were less than 10%.

^bn/e and n/i are the selectivity ratio of K_i (eNOS or iNOS) to K_i (nNOS).

^cL-NNA: L-N^ω-nitroarginine, a positive control.