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. 2010 Nov 10;5(11):e13930. doi: 10.1371/journal.pone.0013930

Figure 3. Effects of CsA on Na/K-ATPase in HK-2 cells.

Figure 3

A–B. Dose- and time-response effects of CsA on Na/K-ATPase activity. CsA inhibited Na/K-ATPase activity at concentrations 10 µM (A). Time course inhibition of Na/K-ATPase activity after 2 h, 4 h, 10 h and 24 h, at 10 µM CsA (B). C: Na/K-β1 stady-state levels after CsA treatment. Effects of different doses of CsA on Na/K-β1 protein expression levels. β-actin was used as a loading control. D–E: Effects of CsA on Na/K-β1 and CypB localization. Immunocitochemistry assays using anti-CypB and anti-Na/K-β1 rabbit polyclonal antibodies were performed on untreated (upper panel) and 10 µM CsA treated (lower panel) HK-2 cells. F–G: Effects of CsA on CypB expression. Intracellular (F) and secreted (G) CypB levels in HK-2 cells treated with different doses of CsA. Ratios between CypB and actin signals in cell lysates have been represented upon quantification. Figures are representative of at least three independent experiments.