Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Nov 10;5(11):e15440. doi: 10.1371/journal.pone.0015440

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Mondal et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A. Interaction of GFP-tagged Rac proteins with GST-GRD in a pulldown experiment. Upper panel (pull down), GFP-fusions pulled down using GST-GRD; lower panel (input) showing whole cell lysates probed for the GFP-fusion proteins. B. Interaction of constitutively activated Racs with GRD in a yeast two-hybrid assay represented in a table. C. GFP-GAPA associates preferentially with activated Rac1a. Filamin is also present in the complex. GFP-GAPA and Filamin were recognized using appropriate antibodies, GST-Rac1a using GST polyclonal antibodies. D. Pulldown of Filamin using GST-Rac1a. Rac1a could pull down GFP-FLN which was recognized by mAb K184-3, GST and GST-Rac1a were visualised by Ponceau S staining.