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. 2010 Oct 19;9:77. doi: 10.1186/1475-2859-9-77

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Copyright ©2010 Fong and Wood; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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IPTG induction of ELP-tagged targets in TB shake flasks. Comparison of IPTG-induced expression (denoted by *) versus leaky expression in TB medium using the BLR (DE3) strain. In each case the overexpressed precursor protein is marked. 1: α-hemoglobin stabilizing protein (EI-AHSP = 73 kDa), 2: β-lactamase (EI-β-lac = 90 kDa), 3: β-galacotosidase (EI-β-gal = 177 kDa), 4: green fluorescent protein (EI-GFP = 88 kDa), 5: glutathione S-transferase (EI-GST = 87 kDa), and 6: maltose binding protein (EI-MBP = 102 kDa).